easy-dhpsf:使用双螺旋PSF拟合3D单分子图像的GUI-开源

上传者: 42157188 | 上传时间: 2023-03-02 10:57:44 | 文件大小: 151KB | 文件类型: ZIP
使用双螺旋点扩散函数(DH-PSF)宽视场显微镜时,这种附带MATLAB的MATLAB例程包可让科学用户测量单个分子的3D位置。 版本2还包含使用本地加权二次3D配准函数促进结合来自两个光谱通道的定位数据的代码特征-通过对明亮的固定荧光发射器(例如,磁珠)进行轴向扫描来校准双高斯估计量-通过进行DH定位来进行模板匹配和随后的双高斯拟合-使用模板匹配分析SM图像的Tiff堆栈,然后进行双高斯拟合以提取分子位置的估计值-通过从控制点对生成局部加权的二次映射函数来注册两个光谱通道

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