UMI-tools:用于处理NGS数据集中的唯一分子标识符的工具

上传者: 42138376 | 上传时间: 2023-06-17 23:15:32 | 文件大小: 15.13MB | 文件类型: ZIP
UMI-tools于17年1月18日发表在(开放获取) 有关完整的文档,请参见 处理唯一分子标识符的工具 该存储库包含用于处理唯一分子标识符(UMI)/随机分子标签(RMT)和单细胞RNA-Seq细胞条形码的工具。 当前有6个命令。 extract和whitelist命令用于准备包含uMI的fastq +/-细胞条形码以进行对齐。 白名单: 建立“真实”细胞条形码的白名单 对于真正细胞条形码的身份未知的基于液滴的单细胞RNA-Seq,这很有用。 然后,白名单可用于过滤提取物(请参见下文) 提炼: 从fastq读取中灵活删除UMI序列。 删除了UMI,并将其附加到读取的名称之后。 任何其他条形码(例如库条形码)都保留在读取中。 也可以按质量或针对白名单过滤读取内容(请参见上文) 其余命令group , dedup和count / count_tab用于使用UMI识别PCR重复

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